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. 2016 May 4;9:100. doi: 10.1186/s13068-016-0514-1

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© Shao et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Design of homologous recombination fragments (a) recombination events for deleting tdk in wild-type strain (b) recombination events for deleting ldh in X20 (c) and recombination events for inserting tdk into X20 (d) P promoter; up upstream; down downstream; int internal