Fig. 3.

Bands observed after reamplification of previous PCR product with the TEM family-specific primers. PCR products were resolved on 2 % agarose gel stained with 1 µg/ml ethidium bromide at 100 V for 30 min. The gel was photographed under UV illumination. Lane M 1 kb plus DNA ladder, Lanes 2, 4, 5, 7, 8, 10 PCR product from showing amplified TEM gene with band position at 720 bp, Lane 6 TEM-3-producing E. coli NCTC 13351, Lanes 1, 3, 9 PCR product with no amplification