Table 1.

Sequences, annealing temperatures and expected product sizes of primer sequences targeting the specified ESBL genes

Gene	Primer	Annealing temp. (°C)	Expected product size (bp)
bla TEM	f: 5′-AAA CGC TGG TGA AAG TA-3′ r: 5′-AGC GAT CTG TCT AT-3′	45	720
bla SHV	f: 5′-ATG CGT TAT ATT CGC CTG TG-3′ r: 5′-TGC TTT GTT ATT CGG GCC AA-3′	60	726
bla CTX-M	f: 5′-GAC GAT GTC ACT GGC TGA GC-3′ r: 5′-AGC CGC CGA CGC TAA TAC A-3′	55	499

Sources Bonomo et al. [22] (bla _TEM), Hanson et al. [23] (bla _CTX-M), Bonomo et al. [24] (bla _SHV)