Table 5.
Donor isolate | Total | Conjugation successful | Percent conjugability (%) |
---|---|---|---|
Klebsiella pneumoniae | 22 | 19 | 86.4 |
Escherichia coli | 18 | 9 | 50.0 |
Enterobacter spp. | 5 | 2 | 40.0 |
Citrobacter spp. | 4 | 1 | 25.0 |
Proteus spp. | 1 | 1 | 100 |
Total | 50 | 32 | 64 |
ESBL-positive study isolates were conjugated to nalidixic acid-resistant E. coli K-12. Selection of transconjugants was done on MacConkey agar (MAST, UK) supplemented with ampicillin and nalidixic acid