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Numbers of detected conjugative TEM genes
| Isolate | TEM | Number that had transferred by conjugation |
|---|---|---|
| Citrobacter spp. | 0 | 0 |
| Enterobacter spp. | 1 | 0 |
| Escherichia coli | 11 | 3 |
| Klebsiella spp. | 12 | 8 |
| Proteus spp. | 1 | 1 |
The TEM primer produced PCR amplification product in DNA extracts from 25 of the ESBL-positive isolates. Verification of which of these genes had been transferred by conjugation was obtained by comparing this data to results of PCR on plasmid-DNA extracts from the transconjugants
