Figure 2. Analytical framework for analysis of endogenous peptides.

(a) Optimized sample preparation protocol for experimental isolation of endogenous peptides from the hypothalamus before mass spectrometric analysis. (b) Computational data analysis was done in the context of orthologous protein groups enabling comparison between species and incorporation of previously annotated peptides from multiple databases and online resources to focus the analysis on a subset of peptides. (c) Generation of LPVs is schematically shown as removal of redundancy and merging of overlapping peptides. Illustrations were generated using images from Servier.com.