Figure 3. Overexpression of NPF3 mimics GA deficiency.

(a) Phenotypes of 3 week-old npf3 mutants and p35S:NPF3-YFP lines compared with WT (Col-0). Bar, 1 cm. (b) Quantification of root and hypocotyl length of 7-day-old seedlings growing on MS media. Values are means±s.e. of 11 seedlings per genotype in two independent experiments n=22. (c) GA₃-Fl accumulation in p35S:NPF3-YFP plants under reduced detector gains, (5 μM GA₃-Fl, 1.5 h treatment). Bar, 50 μm. (d) Graph presenting root elongation on 5 μM paclo, and response to exogenously applied 5 μM GA₃. Shown are means±s.e. of at least 11 seedlings per genotype. The experiment was repeated three times. (e) Representative images of 4 week-old WT and p35S:NPF3-YFP plants grown on soil with or without 10 μM GA₃ spraying, n=12, bar, 1 cm. (f) Hypocotyl length of 2 independent p35S:NPF3-YFP lines and WT (Col-0) seedlings germinated and grown on MS with or without 5 μM GA₄. Values are means±s.e. of 13 seedlings per genotype. * Significantly different relative to respective WT at P≤0.001 by Student t-test.