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. 2016 May 3;7:11415. doi: 10.1038/ncomms11415

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(a) S-Myosin (green) and E-Myosin (green) expression in hindlimb muscles from 2-month-old mice. (b) Quantification of S-Myosin and E-myosin expression in a; n=3. (c) Western blot analysis of S-Myosin and E-Myosin expression in skeletal muscles. GAPDH was used as a loading control; n=6. (d) S-Myosin (green) expression in control and PKO PDGFRβ⁺ cells after 15 days in myogenic differentiation in the presence of saline or exogenous laminin. (e) Participation of TdT-labelled PDGFRβ⁺ cells (red) in the formation of S-Myosin (green)⁺ myofibres after co-culturing with wild-type satellite cells in myogenic medium. Scale bars, 100 μm in a,d and e. **P<0.01; ***P<0.001 (Student's t-test). The results are shown as mean±s.d.