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. 2016 May 5;15:74. doi: 10.1186/s12934-016-0475-x

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© Yang and Nie. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Comparative growths of the engineered strains using acetic acid. The engineered strains E. coli BL21(DE3)/pCOLADUet-1 (a), BL21(DE3)/pYJM60 (b), BL21(DE3)/pYJM62 (c) and BL21(DE3)/pYJM61 (d) grew on different concentrations of acetate. The OD₆₀₀ was measured spectrophotometrically. The experiment was performed in triplicate