Table 1.
Strains and plasmids used in this study
| Name | Relevant characteristics | References |
|---|---|---|
| Strains | ||
| E.coli BL21(DE3) | F− ompT hsdSB (r−Bm−B) gal dcm rne131 λ(DE3) | Invitrogen |
| E.coli DH5α | deoR, recA1, endA1, hsdR17(rk-,mk +), phoA, supE44, λ-, thi-1, gyrA96, relA1 | Takara |
| YJM60 | E.coli BL21(DE3)/pYJM60 | This study |
| YJM61 | E.coli BL21(DE3)/pYJM61 | This study |
| YJM62 | E.coli BL21(DE3)/pYJM62 | This study |
| YJM63 | E.coli BL21(DE3)/pYJM63, pYJM62 | This study |
| YJM64 | E.coli BL21(DE3)/pYJM64, pYJM62 | This study |
| YJM66 | E.coli BL21(DE3)/pYJM66, pYJM14, pYJM62 | This study |
| YJM67 | E.coli BL21(DE3)/pYJM66, pYJM14, pYJM67 | This study |
| Plasmids | ||
| pACYCDuet-1 | P15A (pACYC184), Cmr | Novagen |
| pTrcHis2B | pBR322 origin, Ampr | Invitrogen |
| pCOLADuet-1 | ColA ori, lacI T7lac, Kanr | Novagen |
| pYJM14 | pTrcHis2B carrying ERG12, ERG8, ERG19 and IDI1 from Saccharomyces cerevisiae | [29] |
| pYJM16 | pACYCDuet-1 carrying mvaE and mvaS from Enterococcus faecalis | [28] |
| pYJM60 | pCOLADuet-1 carrying ACS EC from E. coli | This study |
| pYJM61 | pCOLADuet-1 carrying ACS ST from Salmonella typhimurium LT2 | This study |
| pYJM62 | pCOLADuet-1 carrying ACS AP from Acetobacter pasteurianus | This study |
| pYJM63 | pACYCDuet-1 carrying QHS1 from Artemisia annua | This study |
| pYJM64 | pACYCDuet-1 carrying QHS1 from Artemisia annua, GPPS2 from Abies grandis | This study |
| pYJM65 | pACYCDuet-1 carrying QHS1 from Artemisia annua, mvaE and mvaS from Enterococcus faecalis | This study |
| pYJM66 | pACYCDuet-1 carrying QHS1 from Artemisia annua, mvaE and mvaS from Enterococcus faecalis, GPPS2 from Abies grandis | This study |
| pYJM67 | pCOLADuet-1 carrying ACS AP from Acetobacter pasteurianus, nphT7 from Streptomyces sp. strain CL190 | This study |