Figure 2.
Mannose receptor (MR) drives protective T-helper type 1 (Th1) and Th17 cells and vaccine-induced resistance. A and B, Wild-type and MR−/− mice adoptively received 106 CD4+ purified, naive 1807 transgenic (Tg) cells (Thy1.1+) and were or were not vaccinated with 106 heat-killed vaccine yeast. Four weeks after vaccination, mice were challenged intratracheally with 2 × 104 strain 26199 yeast, and the number of activated (CD44+) and cytokine-producing lung CD4+ T cells were enumerated at day 4 after infection. Dot plots show concatenated samples of 4–6 mice/group. The numbers indicate mean values ± standard errors of the mean (SEMs) of activated (CD44hi) endogenous CD4+ and transferred 1807 Tg (Thy1.1+) T cells. Data are representative of 2 independent experiments. *P < .05 vs yeast-stimulated, non–mannan-treated controls. C, Cytokine transcripts in lung homogenates were analyzed at day 4 after infection, and cytokine levels were measured in ex vivo–stimulated splenocytes. D, The lung burden was assessed at day 4 and 2 weeks after infection. Data are the average and SEMs of 3 independent experiments. *P < .05 vs infected wild-type controls. Abbreviations: CFUs, colony-forming units; IFN-γ, interferon γ; IL-17, interleukin 17.