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. 2016 Jan 24;213(11):1809–1819. doi: 10.1093/infdis/jiw030

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© The Author 2016. Published by Oxford University Press for the Infectious Diseases Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Representative flow cytometric gating strategy. Dot plots are shown for cells isolated from the duodenal mucosa (A) and peripheral blood (B). Dead cells, B cells, and monocytes were removed by staining for viability (Vivid), CD19, and CD14 and gating on the negative population. T cells were identified according to the expression of CD3. T cells were classified according to the expression of CD4 and CD8 and the expression of interferon γ (IFN-γ), tumor necrosis factor α (TNF-α), and/or interleukin 2 (IL-2) assessed in nonstimulated and in live-attenuated Salmonella Typhi strain Ty21a (Ty21a)-, influenza virus–, and staphylococcal enterotoxin B (SEB)–stimulated samples.