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Effect of Ang II on the level of ROS in NRK-49F cells. (A) Cells were treated with Ang II for indicated times. DHE assay was used to detect the level of ROS in NRK-49F cells. (B) Cells were treated with Ang II for 24 h. The level of ROS was visualized by DHE fluorescence images. (C, D) P47 was assayed by Western blot. Data are the mean±SEM of 3 experiments. *P<0.05, **P<0.01 compared with control.
