Figure 4.

Effect of hyperoside on LPS-induced nuclear translocation of NF-κB in human RA FLSs. RA FLSs were incubated with LPS (1 μg/mL) for the indicated time with or without the indicated concentration of hyperoside. In coincubation experiments, hyperoside was added to cells 4 h prior to LPS and then treated with LPS for 30 min. (A and B) Representative immunoblot comparing NF-κB subunit p65 to β-actin in RA FLSs after the indicated treatment. Group data showing the normalization of p65 to β-actin as determined in each group from the 3 experiments. (C) Representative immunofluorescence photographs show the location of p65 in RA FLSs. Values are the mean±SEM of three independent experiments. ^**P<0.01 compared with the untreated control. ^##P<0.01 compared with the LPS.