Figure 7.

Therapeutic effect of hyperoside treatment on established arthritis in mice with collagen-induced arthritis (CIA). Following the development of clinical arthritis (average clinical score of 2) on day 31, mice with CIA were randomized to receive injection with either the vehicle or hyperoside (25 mg/kg and 50 mg/kg, respectively). (A) Clinical evidence of arthritis with redness and swelling of the paws involving the tarsus, ankle and toes in the three treated groups. Pictures were taken at day 52. (B) Clinical scores were measured on the indicated days after the primary immunization. (C) Histopathological changes in hyperoside-treated mice with CIA. Hind paws from mice with CIA were obtained on day 52 and were then sectioned and stained with hematoxylin and eosin (H&E) or Safranio O. Photomicrographs are representative of the 3 independent experiments (Original magnification 200 for H&E- and Safranin O–stained sections). Black arrows indicate synovium inflammation. White arrows indicate cartilage damage. Data represent the mean± SEM(n=8 mice per group). ^#P<0.05, ^##P<0.01 compared with the vehicle-treated CIA mice.