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. Author manuscript; available in PMC: 2016 May 5.
Published in final edited form as: Int Rev Cell Mol Biol. 2014;310:89–128. doi: 10.1016/B978-0-12-800180-6.00003-7

Table 3.1.

List of known binding domains on Xin proteins and known Xin-interacting proteins

Interacting protein Domain/motif mapped or unmapped (sequence homology) Common or unique References
Actin filaments (binding and bundling) The Xin repeat region:

  1. aa#89–742 of mXinα; aa#89–738 of XIRP1/hXinα (containing 15 Xin repeats)

  2. aa#308–1306 of mXinβ; aa#537–1535 of XIRP2/hXinβ (containing 28 Xin repeats, from NP_689594.4)

 Common to both Xinα and Xinβ Cherepanova et al. (2006), Choi et al. (2007), Grosskurth et al. (2008), Huang et al. (2006), Pacholsky et al. (2004), van der Ven et al. (2006), and Wang et al. (2010)
β-Catenin Highly conserved β-catenin-binding domain (mapped to aa#535–636 of mXinα) (aa#808–905 of mXinβ, unmapped) May be common to both Xinα and Xinβ Choi et al. (2007) and Grosskurth et al. (2008)
p120-Catenin Multiple p120-catenin-binding sites on mXinα (the strongest binding site locates in the first half of the Xin repeat region aa#68–371, mXinα1R, as detected by cotransfection and Co-IP from CHO cells) May be common to both Xinα and Xinβ Wang et al. (2013b)
Cortactin Multiple cortactin-binding sites on mXinα; one of them locates in aa#1–71 (mXinαNTR), and the strongest binding site locates in the second half of the Xin repeat region aa#364–748, mXinα2R, as detected by cotransfection and Co-IP from CHO cells ? Wang et al. (2013b)
EVH1 (Ena/VASP homology-1) domain-containing family proteins, including Mena, VASP, and Ena-VASP-like (EVL) protein

  1. Mena/VASP-binding domain (M/V-BD), mapped to aa#20–32 of XIRP1 (EDLPLPPPPALED) with N- and C-terminal acidic residues as important determinants for binding affinity

  2. This M/V-BD is highly homologous to the consensus sequence of class I EVH1 ligands (F/W/Y/L)PPPPX(D/E)(D/E) (D/E)

  3. Sequences at the similar position in mXinα (#20–32), mXinβ (#210–222), and hXinβ (#439–451) are EDLSLPHPSAPEG, AQDNGTPSGKMEE, and AQINATSSGMTEE, respectively

 May be unique to XIRP1/hXinα and mXinα Grosskurth et al. (2008) and van der Ven et al. (2006)
Filamin c (Ig domain 20, muscle-specific) Filamin c-interacting region mapped to aa#1685–1843 of XinA large variant from XIRP1/hXinα (aa#1667–1820 of mXinα-a) Unique to the large variant (XinA) of hXinα and mXinα-a Grosskurth et al. (2008) and van der Ven et al. (2006)
Filamin b (aa#2533–2603) Interaction with mXinα, detected by yeast two-hybrid screening unmapped ? Choi et al. (2007)
Tropomyosin (aa#1–284) Interaction with mXinα, detected in yeast two-hybrid screening unmapped ? Choi et al. (2007)
Gelsolin (aa#497–780) Interaction with mXinα, detected in yeast two-hybrid screening unmapped ? Choi et al. (2007)
Vinculin (aa#1–1066) Interaction with mXinα, detected in yeast two-hybrid assay unmapped ? Choi et al. (2007)
α-Actinin Multiple α-actinin-interacting fragments (aa#334–680 and #1340–1644) of myomaxin (mXinβ), as detected by cotransfection and Co-IP from COS cells ? Huang et al. (2006)
KChIP2 (potassium channel-interacting protein 2) Interaction with mXinα, detected by yeast two-hybrid assay unmapped ? Chan et al. (2011)