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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Mol Microbiol. 2015 Oct 14;99(1):88–110. doi: 10.1111/mmi.13218

Figure 2. Wild-type H. pylori strains display diverse cell shape and flagellum morphologies.

Figure 2

(A) Side curvature vs. cell length (μm) for individual bacterial cells imaged using phase contrast microscopy of LSH100 (blue, n=282), B128 (green, n=274), and PMSS1 (orange, n=222) bacteria. Inset panel: TEM images of each. Scale bar = 1 μm. Data combined from two independent cultures for each strain. (B) Representative bacterial centerlines (red dots) for each wild-type strain fitted with a generalized sine function, y=Rsin(2πPx+δ) (black dashed line); R, helical radius; P, helical pitch; δ, phase shift term (δ ≈ π for the bacteria shown). (C–E) Smooth histograms of probability density for cell length (μm) (C), helical radius (μm) (D), and helical pitch (μm) (E) of LSH100 (n=262), B128 (n=272), and PMSS1 (n=215) bacteria. (F) Flagellum number counts measured from SEM or TEM images reported as percent of the total bacterial population examined (n=104–110 bacteria).