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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Nat Protoc. 2016 Apr 7;11(5):872–881. doi: 10.1038/nprot.2016.049

Figure 4.

Figure 4

Sarcomere morphology and expansion of epicardial cells during explant culture. Cultured hearts from cmlc2: actinin3-EGFP fish were fixed at the indicated time point. (Top panels) Heart sections were stained with an anti-Raldh2 antibody to denote epicardial and endocardial cells (red) and DAPI for DNA (blue). Sarcomeric structure (green) was largely maintained in the first 2 weeks, and the epicardial cell population expanded. Cardiac muscle compresses somewhat at day 14, which likely causes tissue shrinkage. (Bottom panels) DAPI (white) staining showed largely normal myocardial nuclei. Necrosis was rare within 14 days of culture.