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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Nat Protoc. 2016 Apr 7;11(5):872–881. doi: 10.1038/nprot.2016.049

Figure 6.

Figure 6

In situ hybridization on sections of ventricular explants after 2 days of culture. raldh2, tbx18 and wt1 were induced during explant culture. For most explants, raldh2 was only detected in the wall (top left), while some explants showed endocardial expression (top right), suggestive of endocardial activation by injury.