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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Nat Protoc. 2016 Apr 7;11(5):872–881. doi: 10.1038/nprot.2016.049

Figure 7.

Figure 7

Ex vivo epicardial regeneration and chamber engraftment. (a) Ventricular epicardium regenerates in a base-to-apex direction (arrows). Hearts from tcf21:NTR; tcf21:nucEGFP adults were incubated with 1 mM Mtz for 24 h. Whole mount images were taken daily. dpi, days post Mtz incubation. (b) A freshly collected outflow tract from a tcf21:nucEGFP fish (green) was grafted to the base of a ventricle from tcf21:NTR fish (red), by 48 h contact in agarose. The ventricle was treated with 1 mM Mtz for 24 h and cultured for 2 more days before engraftment. (c) A non-transgenic donor outflow tract (OFT) was transplanted to the base of an epicardially ablated ventricle. Base-to-apex epicardial regeneration (arrows) was observed from host tissue. dpt, days post-transplantation.