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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Plant Biotechnol J. 2015 Feb 16;13(9):1300–1311. doi: 10.1111/pbi.12349

Figure 3.

Figure 3

Ectopic expression of the T474 mutant receptor down-regulates global protein synthesis in leaves of 10 days (a) old and 28 days old (b) tomato plants. Equal fresh weights of tomato leaves (300 mg) were incubated with 50 μg/mL chloramphenicol and 20 μCi of [35S]methionine for 3 h at room temperature. Incorporation of [35S]Met into protein was measured in the TCA-precipitated total protein (mean ± SD, n = 3, P < 0.05) from wild-type and T474D transgenic lines. Asterisks indicate significant differences from the wild-type control (P < 0.05, Student’s t-test).