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. 2016 Mar 9;3(1):52–61. doi: 10.1002/reg2.50

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© 2016 The Authors. Regeneration published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Immunofluorescence analysis of regenerated A. cahirinus ear punch. (A) Masson's trichrome stain (50x); vertical dotted line indicates original plane of wounding; yellow arrowheads indicate cartilage; green arrowheads indicate muscle; orange arrowheads indicate hair follicles. (B) Incubated with anti‐actin (50x). (C) Probed with anti‐actin (200×). (D) Negative control in which the primary antiserum was omitted (200×). (E) Incubated with anti‐TUJ1 (50x). (F) Probed with anti‐TUJ1 (200×). (G) Negative control stained with secondary antibody only (200×). (H) Merge of B + E + Dapi (5x). (I) Merge of B + E + Dapi (200×). All scale bars 100 μm.