Figure 1. Acyl biotin exchange method used in this study.
A) Flowchart depicting the schematics of the ABE technique. B) Representative Coomassie Blue-stained gel of ABE-treated Toxoplasma gondii protein extract. HA+: Hydroxylamine-treated sample. HA-: Tris-treated control (no hydroxylamine). M: molecular mass reference. Both lanes were dissected into small slices and analyzed by MS (mass spectrometry).