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. 2016 Apr 29;9:2565–2574. doi: 10.2147/OTT.S100468

Figure 5.

Figure 5

STMN1 was a target gene of miR-101.

Notes: (A) The binding sites of hsa-miR-101 of STMN1 mRNA 3′UTR and mutation of STMN1 mRNA 3′UTR. (B) The repression of luciferase activity by STMN1 3′UTR was dependent on miR-101. Mutated STMN1 3′UTR abrogated miR-101 mediated repression luciferase activity in A549 and H1299 cells. (C) Western blot analyzed the protein expression levels of STMN1 after transfecting with miR-101 mimic, miR-101 inhibitor, and negative controls in A549 and H1299 cells. Data presented as mean ± SD. *P<0.05.

Abbreviations: anti-con, the control of anti-STMN1 loss models; con, control; hsa, homo sapien; STMN1, Stathmin1; SD, standard deviation.