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. 2016 May 5;11(5):e0155081. doi: 10.1371/journal.pone.0155081

Fig 2. Cytokine production in splenocytes and BMDC and maturation of BMDC.

Fig 2

Splenocytes (A) and BMDC (B) derived from naive BALB/c mice were stimulated with OLA or cultured with medium alone. IFN-γ, IL-6 and IL-10 levels were determined in supernatants by ELISA. Results show pooled values ± SEM of three to four independent experiments performed with cell suspensions prepared from one individual mouse per experiment. * p < 0.05; ** p < 0.01; *** p < 0.001. BMDC cultured with Pam3Cys, LPS, OLA or medium alone were analysed by flow cytometry for the expression of the maturation markers CD40, CD80, CD86 and MHCII after gating on CD11c+ cells (C). Representative histograms from one out of three repeated experiments.