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. 2016 May 5;11(5):e0154702. doi: 10.1371/journal.pone.0154702

Fig 1. Protein kinase A phosphorylation of recombinantly produced and purified yeast (yCK) and human (hCK) choline kinases.

Fig 1

yCK, hCKα2, and hCKβ were treated (+) with 80 U of PKA catalytic subunit. Unphosphorylated samples are indicated by “−”. Arrows indicate positive phosphoprotein staining for yCK and hCKβ. M: BenchMark™ Protein Ladder. Ten micrograms of the phosphorylated proteins were analyzed by 10% SDS-PAGE. Phosphorylated and total proteins were stained with Pro-Q Diamond phosphoprotein stain and SYPRO® Ruby stain, respectively.