Table 4. Oligonucleotides used in this study.
Oligonucleotides | Sequence (5’ to 3’) |
---|---|
42N truncation CKβ-NdeI-5’ | GAATTCCATATGCGTGACGCCGAGCGCC |
S39ACKβ-AscI-5’ | AAAACGGCGGCGCGCCGCGTCGCTGTCGCGTG |
S40ACKβ-AscI-5’ | AAAACGCCGGCGCGCCTCGGCGCTGTCGCGTGACG |
S42ACKβ-AscI-5’ | AAAACGGCGGCGCGCCTCGTCGCTGGCGCGTGACGCCG |
S39AS42ACKβ-AscI-5’ | AAAACGGCGGCGCGCCGCGTCGCTGGCGCGTGACGCCG |
S40AS42ACKβ-AscI-5’ | AAAACGGCGGCGCGCCTCGGCGCTGGCGCGTGACGCCG |
S39AS40ACKβ-AscI-5’ | AAAACGGCGGCGCGCCGCGGCGCTGTCGCGTGACG |
S39DS40DCKβ-AscI-5’ | AAAACGGCGGCGCGCCGATGATCTGTCGCGTGACG |
CKβ-BamHI-3’ | CGCGGATCCTCAGGATGAGGAGTGGACACTGG |