Skip to main content
. 2016 May 5;12(5):e1006019. doi: 10.1371/journal.pgen.1006019

Fig 1. Transgenic overexpression of LTBP4 in muscle.

Fig 1

(A) Mouse LTBP4 (mLTBP4) was overexpressed in skeletal muscle under control of the human skeletal actin (HSA) promoter. (B) Quadriceps muscle lysates from wildtype and TG+ mice were immunoblotted for the anti-Xpress tag on the LTBP4 transgene demonstrating LTBP4 protein overexpression. (C) Quadriceps muscle lysates similarly immunoblotted using an antibody to LTBP4 to assess overall LTBP4 expression in muscle (arrow). LC, loading control, represent the MemCode staining for actin. (D) Quantification of immunoblots from wildtype (n = 2) and TG+ (n = 3) muscle lysates showed that the LTBP4 TG+ mice express ~7 times the amount of LTBP4 protein of wildtype mice. (E) Individual myofibers were isolated from TG+ and wildtype control mice and incubated with antibodies to LTBP4 under conditions that did not allow antibody permeabilization. LTBP4 localizes sarcolemma in costameric pattern and extracellular matrix in murine muscle, and TG+ mice have qualitatively more LTBP4 protein expression. Scale bar = 30 μm. (F) Co-immunostaining with an anti-laminin antibody (red) demonstrates the extracellular location of LTBP4 (green) and shown in merged image as yellow. Scale bar 50 μm.