Skip to main content
. 2016 May 5;12(5):e1005994. doi: 10.1371/journal.pgen.1005994

Fig 2. Effects of 70-bp repeats on VSG donor selection and switching.

Fig 2

A) Map of BES1 modifications (in comparison to PA) used in analyzed strains illustrate ISceI site introduction with puromycin marker (green), HpaI restriction site location (red) used in Southern blot conformation is indicated along with anticipated sizes following digestion. B) Southern blots of representative clones from each BES1 modified line are shown for HpaI and HpaI/ISceI digestions probed with terminal BES1 VSG (427–2). C) Semi-quantitative RT-PCR of ISCEI enzyme expression for a 5-fold dilution of input RNA under non-inducting (- Dox) and inducing (+ Dox) conditions alongside a tubulin control. D) Heat map showing the number of populations in which clones (n = number of clones analyzed per strain) expressing a specific VSG arose: Lister427 VSG reference number and the predicted genomic locus (BES, MC, or undetermined [UD]) of the VSG are shown together with the number of populations (out of 5 total), in which it arose as depicted by color intensity using the color key (bottom).