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. 2016 May 5;11(5):e0154937. doi: 10.1371/journal.pone.0154937

Fig 4. Comparison of manual counting and automated counting by qBA.

Fig 4

T. (A) Processed images of a P. aeruginosa biofilm Z-stack. (B) Three manually and individually determined bacteria numbers (red line) per layer compared to individual counting by qBA (2D, green line). (B) Three different biofilms of P. aeruginosa were analyzed manually (red line) and by qBA (2D, green line and 3D, black line). In general the cell distribution within those three independent grown biofilms exhibited a minor deviation.