Table 3. Accuracy of cell counting per Z-layer and of the total cell number of simulated 3D biofilms depending on I and P filters.

			I 25		I 17
Z-stack	Z-layer	estimated N	P 9	P 5	P 9	P 5
A	0	10	1.000	1.000	4.600	4.600
	1	90	1.000	1.000	1.489	1.544
	2	845	0.985	1.020	1.015	1.064
	3	2,901	0.862	1.095	0.859	1.100
	4	2,418	0.485	1.209	0.483	1.211
	total	6,264	0.735	1.127	0.750	1.150
B	0	10	1.000	1.000	4.600	4.600
	1	90	1.000	1.000	1.489	1.544
	2	895	0.931	0.987	0.960	1.028
	3	3,872	0.663	0.897	0.659	0.902
	4	4,628	0.255	0.680	0.253	0.681
	total	9,495	0.493	0.801	0.502	0.816