Table 3. Accuracy of cell counting per Z-layer and of the total cell number of simulated 3D biofilms depending on I and P filters.
I 25 | I 17 | |||||
---|---|---|---|---|---|---|
Z-stack | Z-layer | estimated N | P 9 | P 5 | P 9 | P 5 |
A | 0 | 10 | 1.000 | 1.000 | 4.600 | 4.600 |
1 | 90 | 1.000 | 1.000 | 1.489 | 1.544 | |
2 | 845 | 0.985 | 1.020 | 1.015 | 1.064 | |
3 | 2,901 | 0.862 | 1.095 | 0.859 | 1.100 | |
4 | 2,418 | 0.485 | 1.209 | 0.483 | 1.211 | |
total | 6,264 | 0.735 | 1.127 | 0.750 | 1.150 | |
B | 0 | 10 | 1.000 | 1.000 | 4.600 | 4.600 |
1 | 90 | 1.000 | 1.000 | 1.489 | 1.544 | |
2 | 895 | 0.931 | 0.987 | 0.960 | 1.028 | |
3 | 3,872 | 0.663 | 0.897 | 0.659 | 0.902 | |
4 | 4,628 | 0.255 | 0.680 | 0.253 | 0.681 | |
total | 9,495 | 0.493 | 0.801 | 0.502 | 0.816 |