Fig 4. Blood carbon dioxide levels increase significantly under alpha chloralose sedation over the course of 120 minutes.

A) Carbon dioxide levels in arterial blood were significantly higher under alpha chloralose sedation (F_(1,82) = 38.06, p<0.0001, n = 4–19 per time point). Although there was an overall effect of time (F_(5,82) = 4.06, p = 0.002), reflecting increasing CO₂ levels over time, these levels did not differ significantly from baseline over the course of 120 minutes under isoflurane anesthesia (p>0.05). B) After 120 minutes, carbon dioxide levels are significantly higher in alpha chloralose-sedated animals (p = 0.002).