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. 2016 May 5;14(5):e1002450. doi: 10.1371/journal.pbio.1002450

Fig 7. lbe is critical for Ap cluster formation and Tv1 specification.

Fig 7

(B) Eya, Dimm, and Nplp1 expression in control VNCs at stage AFT, showing that Eya is expressed in all four neurons of the Ap clusters and the dAp cells. Dimm is expressed in two out of four Ap cluster cells and the dAp cells, while Nplp1 is expressed in one neuron (Tv1 cell) of each Ap cluster and the dAp cells. (C) In lbe mutants Eya, Dimm and Nplp1 expression is lost in the Ap clusters, whereas their expression in dAp cells is unchanged. (D–E) GFP (lbe(K)-EGFP), Dpn, Col, and Nab expression in NB5-6T at St14 showing that Col expression is lost in lbe mutants while Nab expression is unaffected. (F, G) GFP, Dpn, Lbe, and Nab expression in NB5-6T at St14 in control and col mutants, showing that Lbe expression is not affected in col mutants. (H, I) GFP, Dimm, Nplp1, and Eya expression in the Ap cluster at stage AFT in control, reveals loss of Eya, Dimm, and Nplp1 in lbe mutants. (J, K) GFP, Lbe, Eya, and Nab expression in the Ap cluster at St16 reveals no effect on Lbe expression in col mutants. (L, M) GFP, Lbe, Col, Dpn expression in NB5-6T at St12 and St13 reveals that Lbe is expressed prior to the onset of Col expression. (N, O) Eya, Col, Dimm, and Nplp1 expression at St17 and AFT in the dAp cells of control and lbe mutants, revealing no difference in cell fate specification with respect to Nplp1 expression. Genotypes: (B) OregonR. (C) lbe12C005/Df(lbl-lbe)B44. (D, L, M) lbe(K)-EGFP. (E) lbe(K)-EGFP/+; lbe12C005/Df(lbl-lbe)B44. (F) lbe(K)-EGFP/TTG homozygous. (G) col1/col3; lbe(K)-EGFP/+.