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. 2016 May 5;11(5):e0154855. doi: 10.1371/journal.pone.0154855

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© 2016 Xie et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — Our 898652R715 primer (the first 20 nts in boldface) has its last 7 nts (underlined) annealed to and mis-priming the amplification of the 569382-569448^th-bp region of chr 1 or the 8829-8895^th-bp region of the mtDNA (AY195786.2) with 1-nt mismatch (the lowercase letter “g” in boldface) as the 5’ partner. The 3’ partner is the 8360-8521^st-bp region of the mtDNA (AY195786.2), with our forward primer at the 3’ terminus (898652F4; the underlined 20 nts in boldface) in a reverse-complementary manner. The 3’ partner may also be the 633501-633694^th-bp region of chr 1 with mismatches at 4 nts (lowercase letters in boldface). The two partners overlap at 5’AAATGCCC3’ (underlined, boldfaced, and shaded in grey), which are the 8888-8895^th-bps/8360-8367^th-bps of the mtDNA in AY195786.2.