Fig 5. Two different ending sites (shaded, boldfaced and underlined) of 16S rRNA with the lowercase ‘a” belonging to either the RNA or the poly-A tail appended during polyadenylation.
The boldfaced sequence at the 5’ terminus is the mtR2694 (top panel) or the mtF2581 (bottom panel) primer. The boldfaced sequence after the poly-A tail is the newC primer or part of the newA primer.