Fig 4. A single bisPNA on DNA is resolvable with a ∼6 nm pore.

(a) Population of δG vs. duration for all events in the experiment with DNA/bisPNA (22 nM, 100 mV) with the ∼6 nm pore, overlaid with the DNA/bisPNA data from Fig 3. The event δG histogram (b) and duration histogram (c) shift significantly by using the smaller pore. (d) Schematics of the nanopores and the complex, all sized using a common scale for visual comparison, and a representative event from each experiment (reporting δG, duration). The bisPNA creates a bulge on DNA approximately ∼4 nm in width and length.