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. 2016 May 5;11(5):e0154426. doi: 10.1371/journal.pone.0154426

Fig 5. A single bisPNA on DNA is resolvable with a 17-21 nm pore by adding bulk through bisPEG-PNA linking.

Fig 5

(a) Representative events: DNA/bisPNA (left), and DNA/bisPNA-PEG with up to 3 PEGs on each PNA, and PEG sized 5 kDa (middle) and 10 kDa (right). Molecule depictions show linear PEG and DNA sized to scale for visual comparison. (b) Population of δG vs. duration for all events in each data set, each at 2 nM and 100 mV in 1 M LiCl, with δG histogram (c) and duration histogram (d) shifted by adding PEGs of increasing size. (e) Evolution of the modeled nanopore diameter, using the time history of the open channel conductance (S2 Section), spanning 30 min (DNA/bisPNA), 58 minutes (DNA/bisPNA-PEG 5 kDa) and 40 minutes (DNA/bisPNA-PEG 10 kDa).