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. Author manuscript; available in PMC: 2017 Jan 6.
Published in final edited form as: Neuron. 2015 Dec 10;89(1):113–128. doi: 10.1016/j.neuron.2015.11.025

Figure 2. Rbfox1 regulates mRNA expression: knockdown and microarray analysis.

Figure 2

(A) Experimental flow chart.

(B) Immunoblot showing the knockdown (KD) of Rbfox1 and Rbfox3 proteins in neurons incubated with Accell siRNAs targeting Rbfox1 (siRF1) and Rbfox3 (siRF3). GAPDH is used as loading control.

(C) Histogram of up-regulated (Up) and down-regulated (Dn) exons in KD experiments.

(D) Histogram of up-regulated (Up) and down-regulated (Dn) gene in KD experiments.

(E) Histogram of percentage of genes that contain ≥1 UGCAUG motif conserved in the 3′ UTR in human, mouse, rat, dog, and chicken genomes (UGCAUG_5G), ≥1 UGCAUG motif conserved in human and mouse genomes (UGCAUG_2G), or ≥1 GCAUG motif conserved in human and mouse genomes (GCAUG_2G). *** p < 10−15 by hypergeometric test.

For additional data, see Figures S1 and S2.