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. Author manuscript; available in PMC: 2016 Jul 7.
Published in final edited form as: Cell Rep. 2015 Jun 25;12(1):102–115. doi: 10.1016/j.celrep.2015.05.046

Figure 4. Deficiency of Rictor/mTORC2 does not suppress caspase-1 activation and HK1 expression during NLRP3 inflammasome activation.

Figure 4

(A) Immunoblot analysis for Rictor, HK1, caspase-1 and IL-1β in cell lysates from wild type mice peritoneal macrophages transduced with lentiviruses expressing non-target shRNA (Control shRNA) or two independent shRNA for Rictor (shRictor #1 and #2), and stimulated with LPS and ATP. β-actin served as the standard. (B) ELISA assay for IL-1β, IL-18 and TNF secretion from A. (C) Glucose-6-phosphate, citrate and lactate production assay from wild type peritoneal macrophages transduced with lentiviruses expressing non-target shRNA (Control shRNA) or two independent shRNA for Rictor (shRictor #1 and #2), and stimulated with LPS and ATP.