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. 2016 May 6;6:25340. doi: 10.1038/srep25340

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Copyright © 2016, Macmillan Publishers Limited

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(A) Schematic representation of CaMV35S::ObTLP1 construct used for Agrobacterium-mediated transformation of Arabidopsis following floral dip method. (B) Conformation of the genomic integration of CaMV35S::ObTLP1 construct into independent transgenic lines (TH1-TH4) following PCR analysis using genomic DNA as template. (C) sqRT-PCR analysis confirmed transcript expression of ObTLP1 in leaves of independent transgenic lines (TH1-TH4). AtUBC21 (AT5G25760) amplifications (1082 bp and 448 bp for genomic and cDNA, respectively) were considered as PCR control. ObTLP1 and AtUBC21gels have been run under the same experimental conditions. Full-length gels are presented in Supplementary Fig. S7. (D) Relative transcript level of ObTLP1 in leaves of transgenic lines was determined following qRT-PCR analysis. Data are mean ± s.d. from three biological replicates.