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. 2016 May 6;6:25340. doi: 10.1038/srep25340

Figure 6. ObTLP1-expressing Arabidopsis plants are tolerant to S. sclerotiorum infection.

Figure 6

(A) Leaves of four-week-old ObTLP1-expressing transgenic lines (TH1 and TH2) and vector control plants were inoculated with equal amount of mycelial suspension of S. sclerotiorum as described in Methods. Disease assessment was carried out at different time intervals and photographs were taken. (B) Lesion size was measured at different time intervals in infected leaves. Data are mean ±  s.d. from sixteen plants for each transgenic line. (C) Fungal biomass in infected leaves was quantified by qPCR. Relative level of S. sclerotiorum ITS genomic DNA was determined using Arabidopsis chloroplast-encoded ribulose-1,5-bis-phosphate carboxylase/oxygenase large subunit as reference gene. Data are mean ±  s.d. from three biological replicates. Asterisks indicate statistically significant difference at **P <  0.01 using two-tailed Student’s t-test.