Skip to main content
. 2016 May 6;6:25357. doi: 10.1038/srep25357

Figure 4. Hypoxia induces FoxM1 and RELMα in hypoxic lungs through HIF2α.

Figure 4

(A) Relative FoxM1 mRNA levels (normalized to those of Hprt) in the lungs of control mice exposed to normoxia (n =  4) or hypoxia 10% O2 (n =  6) for 3 days or HIF2α -deficient mice exposed to normoxia (n =  3) or hypoxia 10% O2 (n =  4) for 3 days. (B) Relative RELM-α , RELM-β , EGF and HGF mRNA levels (normalized to that of Hprt) in the lungs of control mice exposed to normoxia (n =  3) or hypoxia 10% O2 (n =  4) for 3 days or HIF2α -deficient mice exposed to normoxia (n =  3) or hypoxia 10% O2 (n =  4) for 3 days. (C) Western blot analysis of RELM-α and β –actin protein levels in the lungs of control or HIF2α -deficient mice exposed to normoxia or hypoxia (10% O2) for 3 days. A representative western blot is shown. (D) RELM-α immunohistochemistry in lung sections of control or HIF2α -deficient mice exposed to normoxia or hypoxia (10% O2) for 3 days. For (A,B) panels values are expressed as mean ±  SEM and n is the number of the animals analysed. Error bars show 95% confidence interval based on duplicated samples. One-way ANOVA Tukey’spost test was used for statistical analysis. *p <  0.05; **p <  0.01; ns (not significant). For (D) panel, scale bar: 50 μ m.