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. 2016 May 6;6:25511. doi: 10.1038/srep25511

Figure 2. Expression of UAS-Cfdp1 transgene activated by different GAL-4 drivers.

Figure 2

(A) Western blotting with anti-CFDP1 antibody of protein estracts from HeLa cells (lane 1) and from salivary glands and brains of elav-GAL4; UAS-CFDP1 larvae (lane 2). Two bands of about 50 kDa and 35 kDa were detected by the anti-CFDP1 in Hela cells, while a single 50 kDa band was found in in elav-GAL4; UAS-Cfdp1 larvae. (B) Overexpression of UAS-Cfdp1 induced by GMR-GAL4; compared to the wild-type eye (left panel), the glazed-like phenotype consist in lack of ommatidia differentiation (left panel); the phenotype shows a 100% penetrance. (C) Overexpression of UAS-Cfdp1 induced by the MS1096-GAL4 driver causes strong reduction of wings portions. The phenotype shows a 100% penetrance. (D) Normal sized brain and imaginal discs of larvae carrying the UAS-Cfdp1 trangene that is not activated (left panel, upper part); severely reduced brain and imaginal discs of larvae expressing UAS-Cfdp1 (left panel, lower part); 60/266 (22%) dying larvae also develop sporadic melanotic masses in their hemocoel (middle panel), while 15/140 (10%) pharate adults show the failure of head eversion (right panel). Among 140 pupae only two vital adults (about 1%) were found. No effects were seen in the GAL4 driver control flies lacking UAS-Cfdp1 transgene.