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. 2016 May 6;6:25511. doi: 10.1038/srep25511

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Copyright © 2016, Macmillan Publishers Limited

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Polytene chromosomes from w, elav-GAL4^[w+]/w; Yeti-GFP^[w+]/UAS-Cfdp1^[w+] female larval progeny were stained with DAPI (blue) and immunostained with both anti-GFP (green) and anti-CFDP1 (red) antibodies. (A,B) YETI-GFP is recruited at DAPI-positive bands; the same patter has been observed when YETI-GFP is expressed in absence of CFDP1⁸; YETI-GFP and CFDP1 proteins mainly colocalize, with CFDP1 showing a slightly broader distribution. (B) A detail of 3R chromosome clearly showing the recruitment of YETI-GFP at DAPI-positive bands. Polytene chromosome bands 93A, 94, 96A, 98B and 99EF are shown as landmarks.