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. 2016 May 6;6:25513. doi: 10.1038/srep25513

Figure 1. ubPCNA accumulation in fission yeast strains defective in specific USP-family ubiquitin proteases.

Figure 1

(a–c) Ubiquitylated PCNA accumulation in exponentially growing (untreated) or methyl methanesulfonate (MMS)-treated single ubp1 to ubp16 deletions in S. pombe. Wild-type and single mutant cells exponentially grown at 32 °C were treated for 3 hours with 0.01% MMS. Western blotting was used to analyse TCA-cell extracts for PCNA ubiquitylation, which was quantified and plotted. Cell extracts were resolved in 12% polyacrylamide gels and immunoblotted with affinity purified rabbit α -PCNA antibodies or α -tubulin (loading). Single ubp1 to ubp16 deletions in S. pombe are indicated by numbers. (d) Graph of mono-ubiquitylated PCNA accumulation in untreated and 0.01% MMS-treated single ubp1–16 deletions in S. pombe. Average values of the relative levels of mono-ubiquitylated PCNA from two independent assays are plotted. Error bars were calculated from two independent experiments and indicate the standard deviation.