Figure 2. D3T inhibits UV-induced RPE cell apoptosis.

APRE-19 cells (A–F) or primary murine RPE cells (G) were irradiated with UV (30 mJ/cm²) with or without D3T (50 μM, 30 min pretreatment), cells were further cultured for indicated time. Cell apoptosis was tested by PI-Annexin V FACS assay ((A–C), for ARPE-19 cells) or histone-DNA apoptosis ELISA assay (F,G). Caspase-9 activity ((D) for ARPE-19 cells) and MMP reduction ((E) for ARPE-19 cells) were also shown. Experiments were repeated three times to insure consistency of results. *p < 0.05 (B–G).