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. 2016 May 6;6:25569. doi: 10.1038/srep25569

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Copyright © 2016, Macmillan Publishers Limited

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Immunohistochemistry for the proximal epithelial markers CGRP (a,b, i,j), β-tubulin IV (c,d,k,l), and CC10 (e,f,m,n) in E18.5 Creb1^EpKO and Creb1^MesKO lungs. Immunohistochemistry for the distal epithelial (type-II AEC) marker ProSPC (g,h,o,p) in E17.5 Creb1^EpKO and E18.5 Creb1^MesKO lungs. No differences in expression or localization was observed for proximal or epithelial markers. Boxed areas are magnified either in insets (CGRP, ProSPC) or below the main image (β-tubulin IV, CC10), while arrowheads indicate marker-positive cells. All images are representative of at least three animals per genotype. Scale bars: a-f, i-n; 180 μm. g,h,o,p; 90 μm.