Figure 4. Lack of compensatory Crem/Atf1 upregulation in Creb1^EpKO and Creb1MesKO lungs.

(a) qPCR analysis of Crem and Atf1 in E18.5 E18.5 Creb1^EpKO (n = 7) and Creb1^MesKO lungs (n = 6). (b,c) Haematoxylin and eosin-stained tissue sections from E18.5 control (Creb1^MesKO; Crem^+/+) and Creb1^MesKO; Crem^−/− lungs. No major difference in lung morphology was observed. Error bars represent SEM. Single asterisk (*) indicates p < 0.05, while double asterisk (**) indicates p < 0.01. White bars: Controls, Black bars: Conditional Creb1 deletions. All images are representative of at least three animals per genotype. Scale bars: 180 μm.