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. 2016 May 6;6:25569. doi: 10.1038/srep25569

Figure 5. Loss of Creb1 does not impair GC signaling in the fetal lung.

Figure 5

(a,b) Haematoxylin and eosin-stained tissue in transverse sections through lower thoracic vertebrae in E18.5 control and Creb1fl−/− mice. Adrenal gland proportions appear comparable between control and Creb1fl−/− mice. (c,d) Immunohistochemistry for GR in E18.5 control and Creb1fl−/− lungs. (e) Measurement of plasma corticosterone in E18.5 Creb1+/+ (white bars), Creb1+/− (grey bars) and Creb1fl−/− (black bars) mice. Error bars represent SEM. All images are representative of at least three animals per genotype. Ad: adrenal gland, Li: liver, Sp: spleen, St: stomach. Scale bars: a, b: 360 μm, c, d: 90 μm.